Fig. 2

Regulation of P2X7 expression in microglia. a mRNA levels of Nos2, Arg1, and P2rx7 in mouse microglia under indicated conditions (100 ng/ml LPS plus 10 ng/ml IFNγ, 10 ng/ml IL4 plus 10 ng/ml IL13, or 100 ng/ml LPS) for 24 h were evaluated by RNA-seq. Data are shown as mean + SD, n = 4. b P2rx7 mRNA levels in mouse microglia exposed to 100 ng/ml LPS plus 10 ng/ml IFNγ, 10 ng/ml IL4 plus 10 ng/ml IL13, 100 ng/ml LPS, or 380 μM BzATP at indicated time durations were measured by quantitative real-time PCR. P2rx7 mRNA level was normalized to Gapdh and presented as fold change compared to vehicle. All experiments were carried out in triplicate and repeated twice independently. Statistical analysis was performed by comparing each condition with vehicle. One-way ANOVA followed by Dunnett’s post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001