Fig. 5

Kv1.3 channels regulate TAP1, EHD1, GABPA, and IL1B expression in primary murine microglia. a Experimental plan for in vivo studies: adult C57BL/6 mice received four daily IP doses of PBS, ShK-223, LPS, or LPS+ShK-223, and brain mononuclear cells were isolated for flow cytometry from one hemisphere and qRT-PCR studies from the other hemisphere (n = 3, mice/group). b Results from flow cytometric studies measuring intracellular EHD1 protein expression in freshly isolated CNS MPs (gated first on live cells, followed by CD11b⁺ CD45^low and CD11b⁺ CD45^high populations). At least 10,000 live CNS MPs were counted per sample. Left: Example of flow cytometric histograms comparing EHD1 expression in CD11b⁺ CD45^low microglia from one mouse from each treatment group. Right: Quantitative analysis of EHD1⁺ cells in CD11b⁺CD45^low and CD11b⁺CD45^high populations. c Results from pRT-PCR studies measuring mRNA expression of TAP1, EHD1, GABPA, and IL1B in CNS MPs. For these studies, all CNS MPs isolated from one hemisphere were used for RNA extraction in Trizol, followed by cDNA preparation followed by quantitative PCR. HPRT was used as the housekeeping gene. Data normalized to HPRT were then normalized to PBS-treated control samples (*p < 0.05, **p < 0.01, ***p < 0.005).