Fig. 2

Microglia exhibit small transcriptional responses to IL-1β. a Representative images of NF-κB immunofluorescence in CX3CR1-YFP-Cre primary microglia that were treated with PBS, IL-1β (50 ng/mL), PBS + 0.1% BSA, or LPS (10 ng/mL) for 30 min. Green = YFP, red = p65 NF-kB, blue = DAPI. Scale bar = 50 μm. Arrows identify cells with nuclear NF-κB. b, c Inflammatory gene expression in primary microglia from WT and MyD88KO mice that were treated with PBS or IL-1β (50 ng/mL) for 4 h. Data are representative of two independent experiments. n = 4 to n = 7 per group. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT PBS, #p < 0.05 vs. WT IL-1β. d, e. Luciferase activity in NF-κB Luc SIM-A9 cells that were treated with PBS, IL-1β (50 or 100 ng/mL), PBS + 0.1% BSA, or LPS (100 ng/mL) for 6 h. Luminescence was normalized to total protein content. Data from two independent experiments were combined. n = 7 to n = 12 per group. Data are expressed as mean ± SEM. ***p < 0.001 vs. PBS/BSA group