Fig. 3

Monocyte-derived macrophages in cerebrospinal fluid (CSF) infiltrate ischemic hemisphere after cortical stroke. a–c Photomicrographs showing representative images of GFP+ monocyte-derived macrophages (MDMs) in ischemic hemisphere. Monocytes were injected into CSF of ipsilateral (a, b) or contralateral (c) lateral ventricle 1 day after stroke, and the animals were analyzed 3 days later. Arrows depict GFP+ MDMs (a, c upper image) and, at high magnification, round MDMs (b, c lower image), in area around ischemic core. Arrow heads indicate GFP+ MDMs located between lateral ventricle and ischemic core (a, c upper image) and, at high magnification, ramified MDMs in area around ischemic core (b, c lower image). d Photomicrographs showing confocal images illustrating colocalization of GFP with M2-like marker CD206 and M1-like marker CD16/32. Arrows indicate GFP+ MDMs (d). Scale bar 200 μm (a, c upper image), 50 μm (b, c lower image), 15 μm (d). LV lateral ventricle