Fig. 10

Plus cells are enriched in macrophage makers while Minus cells preferentially express microglial cell markers. Three weeks after adoptive transfer, retinal single-cell suspensions were analyzed by flow cytometry and sorted into three different cell populations: MHC class II⁺CD45⁺CD11b⁺Ly6C⁺ (Plus or P), MHC class II⁺CD45⁺CD11b⁺Ly6C⁻ (Minus or M), and MHC class II⁺CD45⁻CD11b⁻Ly6C⁻ cells (Non-hematopoietic or NH). a Left: heatmap displaying the gene expression profiles for genes known to be expressed by macrophages. Blind clustering analysis leads to clear separation of the nine samples into three cell populations. Right: boxplot representation of the mean expression of macrophage markers by each cell population. b Left: heatmap displaying the gene expression profiles for genes known to be expressed by microglia. Blind clustering analysis leads to clear separation of the nine samples into three cell populations. Right: boxplot representation of the mean expression of microglial cell markers by each cell population. Each row represents an individual gene and each column a sample from a pool of three mice. Statistical analysis was performed using t tests, *p value <0.05, **p value <0.01