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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis

Fig. 10

Plus cells are enriched in macrophage makers while Minus cells preferentially express microglial cell markers. Three weeks after adoptive transfer, retinal single-cell suspensions were analyzed by flow cytometry and sorted into three different cell populations: MHC class II+CD45+CD11b+Ly6C+ (Plus or P), MHC class II+CD45+CD11b+Ly6C (Minus or M), and MHC class II+CD45CD11bLy6C cells (Non-hematopoietic or NH). a Left: heatmap displaying the gene expression profiles for genes known to be expressed by macrophages. Blind clustering analysis leads to clear separation of the nine samples into three cell populations. Right: boxplot representation of the mean expression of macrophage markers by each cell population. b Left: heatmap displaying the gene expression profiles for genes known to be expressed by microglia. Blind clustering analysis leads to clear separation of the nine samples into three cell populations. Right: boxplot representation of the mean expression of microglial cell markers by each cell population. Each row represents an individual gene and each column a sample from a pool of three mice. Statistical analysis was performed using t tests, *p value <0.05, **p value <0.01

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