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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: MHC class II expression and potential antigen-presenting cells in the retina during experimental autoimmune uveitis

Fig. 8

During EAU, retinal hematopoietic MHC class II+ cells comprise both Ly6C+ and Ly6C cells. Three weeks after adoptive transfer, retinas were carefully dissected, cut into small pieces, and dissociated by enzymatic digestion. Naive eyes were used as control. The single-cell suspensions, excluding dead cells (DAPI+) were analyzed by flow cytometry for MHC class II, CD45, CD11b, and Ly6C expression using fluorochrome-conjugated-specific antibodies. Data are from one representative mouse out of five independent experiments involving 7 naive and 12 EAU-independent animals. a Naive retina. Left: total retinal cells. Middle: gated on MHC class II+ cells (red gate). Right: gated on hematopoietic MHC class II+ CD45+CD11b+ cells (orange gate). b Adoptive transfer uveitis (clinical grade 4). Left: total retinal cells. Middle: gated on MHC class II+ cells (red gate). Right: gated on hematopoietic MHC class II+ CD45+CD11b+ cells (orange gate)

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