Fig. 1
Potentiation of proton-gated currents by PAR2-AP in CHO cells co-expressing ASIC3 and PAR2. a Representative traces show currents evoked by a pH 6.6 acidic solution for 5 s in CHO cells co-expressing ASIC3 and PAR2. The proton-gated current could be blocked by 500 nM APETx2, an ASIC3 inhibitor. b The sequential current traces illustrate the potentiation of proton-gated currents by different concentrations of PAR2-activating peptide (PAR2-AP: 2-furoyl-LIGRLO-NH2, 10−9–10−4 M). Representative currents were recorded for more than 60 min in a cell with membrane potential clamped at −60 mV. PAR2-AP was pre-applied to external solution for 1 min. c The graph shows PAR2-AP increased the peak amplitude of proton-gated currents in a concentration-dependent manner with an EC50 of 2.9 × 10−7 M. Each point represents the mean ± SEM of 8 to 10 cells