Fig. 4

IL-27 expression in MS plaques. a A chronic active lesion of a MS patient autopsy was stained for IL-27 using anti-EBI3 antibody visualized with DAB. The dashed line indicates the lesion border. b Enlarged detail crop from square region in a. c Representative fluorescence immunohistochemistry image of a biopsy showing an early active MS lesions co-immunostained for EBI3 (green) and astrocytes (GFAP, red). Nuclei were counterstained using DAPI (blue). Arrowheads in the merged image indicate EBI3, GFAP double positive cells. Scale bars: a = 200 μm; b = 40 μm; c = 100 μm