Fig. 7
From: Sexually divergent induction of microglial-associated neuroinflammation with hippocampal aging
qPCR confirmation of differential sex- and age-related hippocampal gene expression. Selected microglial ligands (a), effectors (b), and receptors (c) targets identified in the microarray study were confirmed by gene-specific qPCR. Data is scaled to a mean value of 1 for young males. Boxes boundaries are the 25th and 75th percentiles, with median denoted by the bar and error bars at the 10th and 90th percentiles. Two-way ANOVA (age × sex), ***p < 0.001, **p < 0.01, *p < 0.05 Student–Newman–Kuels pairwise post hoc, n = 7–8/group. ANOVA values are presented in the text. Solid comparison lines denote age-related changes with a sex and dashed comparison lines are sex-related differences within an age. d A selection of genes with alternate expression parameters were also confirmed