Fig. 4

Effect of FTY720 treatment on the number of NG2⁺ cells at the level of the dorsal hippocampus. a Schematic diagram of measured areas in the ipsilateral hemisphere (dorsal hippocampus level), in hilus (H), fimbria (F) and corpus callosum (CC). b Representative Z-stack maximal projection of immufluorescence staining for BrdU and NG2 in the (i) hilus, (ii) fimbria and (iii) corpus callosum (KA + FTY720 group). Scale bar: 50 μm. c–j Quantitative analysis of BrdU⁺ and NG2⁺/BrdU⁺ cells. NG2 and BrdU colocalization was determined examining three-dimensional orthogonal reconstruction of confocal layers (ImageJ software). Data are presented as c–f mean of positive cells per mm² ± SEM and g–j percentage of NG2⁺ cells over BrdU⁺ total cells per mm². Statistical analysis: unpaired, two-tailed Student t test; *p < 0.05, **p < 0.01. N = 4 animals for each experimental group