Fig. 5

Fractalkine does not protect astrocytes from low continuous H₂O₂ exposure. Cerebellar slices were pre-treated with fractalkine (CX3CL1; 100 or 10 ng/ml) for 1 h prior to addition of glucose oxidase (GOX; 1:100,000) and catalase (CAT; 1:500,000) in order to generate low levels of H₂O₂ continuously. a Images are representative of five separate experiments. Confocal images taken at × 10 magnification. Scale bar, 100 μm. Molecular layer (ML) and white matter tract (WMT) labelled. b, c Graph shows a significant decrease in vimentin fluorescence when treated with GOX-CAT. Fractalkine treatment does not prevent this astrocyte cell death. Data expressed as mean ± SEM; Student’s t test, ^### p < 0.001; and one-way ANOVA with Tukey’s post hoc test