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Table 2 Gene primers tested in comparative RT-PCR experiments

From: Age exacerbates microglial activation, oxidative stress, inflammatory and NOX2 gene expression, and delays functional recovery in a middle-aged rodent model of spinal cord injury

Gene Sense Antisense
CD86 5′-TGCTCATCTAAGCAAGGATACCCG-3′ 5′-CGACTCGTCAACACCACTGTCCTG-3′
CD206 5′-GGATTGTGGAGCAGATGGAAG-3′ 5′-CTTGAATGGAAATGCACAGAC-3′
p22PHOX 5′-GACGCTTCACGCAGTGGTACT-3′ 5′-CACGACCTCATCTGTCACTGG-3′
p47PHOX 5′-CAGAATGTTGCCTGGTTG-3′ 5′-GTCCCCTCCCTTAGATGA-3′
gp91PHOX 5′-CTTCACACGGCCATTCACAC-3′ 5′-GTCATAGGAGGGTTTCCGGC-3′
TNFα 5′-GGCAGCCTTGTCCCTTGAAGAG-3′ 5′-GTAGCCCACGTCGTAGCAAACC-3′
NFκB 5′-GTGCAGAAAGAAGACATTGAGGTG-3′ 5′-AGGCTAGGGTCAGCGTATGG-3′
iNOS 5′-CAGCCCTCAGAGTACAACGAT-3′ 5′-CAGCAGGCACACGCAATGAT-3′
Arg-1 5′-CAGTATTCACCCCGGCTACG-3′ 5′-GCCTGGTTCTGTTCGGTTTG-3′
CD68 5′-TGTACCTGACCCAGGGTGGAA-3′ 5′-GAATCCAAAGGTAAGCTGTCCGTAA-3′
IL10 5′-CAAGGAGCATTTGAATTCCC-3′ 5′-GGCCTTGTAGACACCTTGGTC-3′
GAPDH 5′-GCTGGTCATCAACGGGAAA-3′ 5′-ACGCCAGTAGACTCCACGACA-3′
  1. Table provides sense and antisense sequences for each gene tested in this study. Genes investigated that showed no significant difference by age in either naïve or injured animals were omitted from results