Fig. 3

The effects of SAM and NaHS administration on neutrophil infiltration and microglia accumulation at 1 day after ICH. a Representative photographs of immunofluorescence staining of MPO immuno-labelled neutrophils (green) and Iba1 immuno-labelled microglia (green) in the perihaematomal area in the vehicle, SAM and NaHS groups at 1 day after ICH. b, c Quantitative analysis of MPO and Iba1-positive cells in the perihaematomal region in the vehicle, SAM and NaHS groups at 1 day after ICH. SAM and NaHS treatment significantly reduced the number of MPO-positive and Iba1-positive cells in the perihaematomal area in the ICH-treated groups compared to the vehicle group. d, e Representative bands and quantitative analysis of MPO and Iba1 protein levels in the perihaematomal tissues in the vehicle, SAM and NaHS groups at 1 day after ICH. MPO and Iba1 protein levels in the ipsilateral hemisphere were markedly reduced after SAM and NaHS treatment in the ICH-treated groups compared to the vehicle group. The relative densities of each protein have been normalized against those of the vehicle group. For western blotting, n = 6 rats per group; for others, n = 4 rats per group. Scale bar = 25 μm. Data are presented as the mean ± SEM. #p < 0.05 vs. vehicle. MPO myeloperoxidase, SAM S-adenosyl-l-methionine, ICH intracerebral haemorrhage