Table 2 Mouse target sequences used to create Custom CodeSet for nCounter Assay

Aif1

NM_019467.2

CTGGAGCAGCCTGCAGACTTCATCCTCTCTCTTCCATCCCGGGGAAAGTCAGCCAGTCCTCCTCAGCTGCCTGTCTTAACCTGCATCATGAAGCCTGAGG

Arg1

NM_007482.3

GTACATTGGCTTGCGAGACGTAGACCCTGGGGAACACTATATAATAAAAACTCTGGGAATTAAGTATTTCTCCATGACTGAAGTAGACAAGCTGGGGATT

Casp1

NM_009807.2

GACAATAAATGGATTGTTGGATGAACTTTTAGAGAAGAGAGTGCTGAATCAGGAAGAAATGGATAAAATAAAACTTGCAAACATTACTGCTATGGACAAG

Ccl22

NM_009137.2

CCAAGAATCAACTTCCACCCCTCTTCAACCACATGCTAGGGTCTTTTACTTTCTCTGCCCCACACCTTTGACTCCTTGCCTGTGTAGCTGATAGTCGAAG

Cd163

NM_053094.2

TCACGGCACTCTTGGTTTGTGGAGCCATTCTATTGGTCCTCCTCATTGTCTTCCTCCTGTGGACTCTGAAGCGACGACAGATTCAGCGACTTACAGTTTC

Cd68

NM_009853.1

GCTCCCTGTGTGTCTGATCTTGCTAGGACCGCTTATAGCCCAAGGAACAGAGGAAGACTGTCCTCACAAAAAGGCCGTTACTCTCCTGCCATCCTTCACG

Cx3cr1

NM_009987.3

TATGCTTTGGTGTTGGTCTGTATTTCCCGCTGTCTCGGGTCACATGGTTAAGCGTGCCTAGAGTGTGTCTATCCCACTTGTAATTCTGTCAATAAACATT

Cybb )

NM_007807.2

ACAGAAGACTCTGTATGGACGGCCCAACTGGGATAACGAGTTCAAGACCATTGCAAGTGAACACCCTAACACCACAATAGGCGTTTTCCTGTGTGGCCCT

Fcgr1a

NM_010186.5

GAGACAGTTCCACACAATGGTTTATCAACGGAACAGCCGTTCAGATCTCCACGCCTAGTTATAGCATCCCAGAGGCCAGTTTTCAGGACAGTGGCGAATA

Fcgr2b

NM_001077189.1

TTGGTTCCCAATGGTTGACTGTACTAATGACTCCCATAACTTACAGCTTCCCAACTCAAGACTCTTCTGCTATCGATCCACACTGCCACTAAAATTAATC

Fcgr3a

NM_010188.5

TCTGACCTCCACCATCCACCATGGCAGGTGCACACAATAAATTAAAATGTCATGTATATTTTTAAACAAGAGACAGGGGCAGGCTAAGGGTTGATGGCAT

Gusb

NM_010368.1

AATACGTGGTCGGAGAGCTCATCTGGAATTTCGCCGACTTCATGACGAACCAGTCACCGCTGAGAGTAATCGGAAACAAGAAGGGGATCTTCACTCGCCA

Hprt

NM_013556.2

TGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTTCCGGAGCGGTAGCACCTCCTCCGCCGGCTTCCTCCTCAGACCGCTTTTTGCCGCGA

Ifng

NM_008337.1

CTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCAGCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAG

Ifngr1

NM_010511.2

AAGCATAATGTTACCTAAGTCCTTGCTCTCTGTGGTAAAAAGTGCCACGTTAGAGACAAAACCTGAATCGAAGTATTCACTTGTCACACCGCACCAGCCA

Ifngr2

NM_008338.3

CATCCTGATTCCGTTGGGCATCTTCGCATTGCTGCTCGGCCTGACGGGCGCCTGCTTCACCCTGTTCCTCAAATACCAAAGCCGAGTGAAGTACTGGTTT

Il10

NM_010548.1

GGGCCCTTTGCTATGGTGTCCTTTCAATTGCTCTCATCCCTGAGTTCAGAGCTCCTAAGAGAGTTGTGAAGAAACTCATGGGTCTTGGGAAGAGAAACCA

Il10ra

NM_008348.2

TGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGACAGAACTGCCAAGCCCTTCCTATGTGTGGTT

Il10rb

NM_008349.5

CTTTACACCTGCGTTTCTCAGCCCCACAAATTGAGAATGAGCCTGAGACGTGGACCTTGAAGAACATTTATGACTCATGGGCTTACAGAGTGCAATACTG

Il13ra1

NM_133990.4

CTCAAACCGACCGACATAATATTTTAGAGGTTGAAGAGGACAAATGCCAGAATTCCGAATCTGATAGAAACATGGAGGGTACAAGTTGTTTCCAACTCCC

Il1b

NM_008361.3

GTTGATTCAAGGGGACATTAGGCAGCACTCTCTAGAACAGAACCTAGCTGTCAACGTGTGGGGGATGAATTGGTCATAGCCCGCACTGAGGTCTTTCATT

Il1r1

NM_001123382.1

CTTCTTCGGAGTAAAAGATAAACTGTTGGTGAGGAATGTGGCTGAAGAGCACAGAGGGGACTATATATGCCGTATGTCCTATACGTTCCGGGGGAAGCAA

Il1rn

NM_031167.5

CAACCAGCTCATTGCTGGGTACTTACAAGGACCAAATATCAAACTAGAAGAAAAGATAGACATGGTGCCTATTGACCTTCATAGTGTGTTCTTGGGCATC

Il4

NM_021283.1

TGCTTGAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCGATAAGCTGCACCATGAATGAGTCCA

IL4ra

NM_001008700.3

CCCACAGCAGTGCTGACGTTCCTAAGTCCTGGGCTTTCCTAGCTGATGTTGTCCTACCTACTCAGTCCCATTTTGTCCACCGAATAGACCTGTCACTCAA

Il6

NM_031168.1

CTCTCTGCAAGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTCACAAGTCCGGAGAGGAGACTTCACAG

Itgam

NM_001082960.1

ATCCCTGTTCAGATCAACAATGTGACCGTATGGGATCATCCCCAGGTCATCTTCTCCCAGAACCTCTCAAGTGCCTGTCACACTGAGCAGAAATCCCCCC

Kcna2

NM_008417.4

GTTAACTGATGTCTGATTGAAGCCTACTAATGTACTCACAGCTCAACAGGACTGATGCAGATGTTGCATAATAGCCTGCATTGTAGTCAGTGTTCTACAG

Kcna3

NM_008418.2

CTGTTGGTTATGGTGATATGCACCCAGTGACCATAGGAGGCAAGATTGTGGGCTCTCTTTGTGCCATCGCAGGTGTCTTGACCATTGCATTGCCAGTTCC

Kcna5

NM_145983.2

AAAAAGTATCGCATTCCATGACGCAGGAGCCGTTGAAGTGGTGAGCATTCACTGTAAGATGGATGTATTCATAGCCAGTTTTCTATACCCAGCAGAGGGA

Kcnj2

NM_008425.4

CTTAAGGCGAGAATCGGAGATATGACTGGCTGATTCCGTCTTTGGAATACTTACTTTGCTACACAGCCTGACGTTGGTCAGAGGTCCGAGACAGTTATAC

Mrc1

NM_008625.1

GTTCCGAAATGTTGAAGGGAAGTGGCTTTGGTTGAACGACAATCCTGTCTCCTTTGTCAACTGGAAAACAGGCGATCCCTCTGGTGAACGGAATGATTGT

Msr1

NM_001113326.1

GATTTCGTCAGTCCAGGAACATGGGAATTCACTGGATGCAATCTCCAAGTCCTTGCAGAGTCTGAATATGACACTGCTTGATGTTCAACTCCATACAGAA

Myc

NM_010849.4

CCCTCAACGTGAACTTCACCAACAGGAACTATGACCTCGACTACGACTCCGTACAGCCCTATTTCATCTGCGACGAGGAAGAGAATTTCTATCACCAGCA

Ncf1

NM_001286037.1

ACCATCCGCAACGCACAGAGCATCCACCAGCGTTCTCGGAAGCGCCTTAGCCAGGACACCTATCGCCGCAACAGCGTCCGATTCCTGCAGCAGCGCAGAC

Nfkbia

NM_010907.1

GTCAGAATTCACAGAGGATGAGCTGCCCTATGATGACTGTGTGTTTGGAGGCCAGCGTCTGACATTATAAGTGGAAAGTGGCAAAAAAGAATGTGGACTT

Nos2

NM_010927.3

CCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCCCTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCC

Nox1

NM_172203.1

CTCCAAACATGACAGTGATGTATGCAGCATTTACCAGTATTGCTGGCCTTACTGGAGTGATTGCCACTGTAGCTTTGGTTCTCATGGTAACGTCAGCTAT

Nox4

NM_015760.4

TCCCAGAAAGCTTCTCTTCACAACCATTCCTGGTCTGACGGGTGTCTGCATGGTGGTGGTATTGTTCCTCATGGTTACAGCTTCTACCTACGCAATAAGA

Nr3c1

NM_008173.3

ACCAGGATTCAGAAACTTACACCTGGATGACCAAATGACCCTTCTACAGTACTCATGGATGTTTCTCATGGCATTTGCCCTGGGTTGGAGATCATACAGA

P2rx7

NM_001038887.1

CTGGAGGAACTGGAAGTTAACCGTTCCTGCTGAGAAATCGGTGTGTTTCCTTTGGCTGCTCCTAGGTGAGGGTTTGCTGTGGTCTAGCCTGGGAAGTAGG

P2ry12

NM_027571.3

GATCACCCAGGTTCTCTTCCCATTGCTGTACACCGTCCTGTTCTTTGCTGGGCTCATCACGAACAGCTTGGCAATGAGGATTTTCTTTCAGATCCGCAGT

P2ry2

NM_008773.3

TAGCCATTTTGTGGCTTACAGCTCCGTCATGCTGGGTCTGCTTTTTGCTGTGCCCTTTTCCGTAATCCTGGTCTGTTACGTGCTTATGGCCAGGCGGCTG

Pparg

NM_011146.1

ACCAAGTGACTCTGCTCAAGTATGGTGTCCATGAGATCATCTACACGATGCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATCTCAGAGGGCCAAGG

Ptgs2

NM_011198.3

CCATCAGTTTTTCAAGACAGATCATAAGCGAGGACCTGGGTTCACCCGAGGACTGGGCCATGGAGTGGACTTAAATCACATTTATGGTGAAACTCTGGAC

Ptk2b

NM_001162365.1

CTTCCGCCGCTTCACAACCGCCAGTGATGTCTGGATGTTTGCTGTATGCATGTGGGAGATCCTCAGCTTTGGGAAGCAGCCTTTCTTCTGGCTCGAAAAT

Ptpn6

NM_013545.2

GACCGAGGCCCAGTACAAGTTTATTTACGTGGCCATTGCCCAGTTCATCGAAACGACCAAGAAGAAACTGGAGATCATACAATCCCAGAAGGGCCAGGAG

Retnla

NM_020509.3

GAATACTGATGAGACCATAGAGATTATCGTGGAGAATAAGGTCAAGGAACTTCTTGCCAATCCAGCTAACTATCCCTCCACTGTAACGAAGACTCTCTCT

Socs1

NM_009896.2

CAGCTTGTGTCTGGGGCCAGGACCTGAATTCCACTCCTACCTCTCCATGTTTACATATTCCCAGTATCTTTGCACAAACCAGGGGTCGGGGAGGGTCTCT

Socs3

NM_007707.2

CCGCGACAGCTCGGACCAGCGCCACTTCTTCACGTTGAGCGTCAAGACCCAGTCGGGGACCAAGAACCTACGCATCCAGTGTGAGGGGGGCAGCTTTTCG

Tgfb1

NM_011577.1

GGAGTTGTACGGCAGTGGCTGAACCAAGGAGACGGAATACAGGGCTTTCGATTCAGCGCTCACTGCTCTTGTGACAGCAAAGATAACAAACTCCACGTGG

Tgfbr1

NM_009370.2

TCAGAAGTAGTGGCCAGCTGTGTCTCTAGTAGGACAGTAAAGGCATGAAGCTCAGCCTGTAATCCTGCTACTACAGTAGTACTCCAGAAGTGCCTTGAGG

Tgfbr2

NM_009371.2

TGTGCAAGTTTTGCGATGTGAGACTGTCCACTTGCGACAACCAGAAGTCCTGCATGAGCAACTGCAGCATCACGGCCATCTGTGAGAAGCCGCATGAAGT

Tlr2

NM_011905.2

GCAGGCGGTCACTGGCAGGAGATGTGTCCGCAATCATAGTTTCTGATGGTGAAGGTTGGACGGCAGTCTCTGCGACCTAGAAGTGGAAAAGATGTCGTTC

Tlr4

NM_021297.2

AACGGCAACTTGGACCTGAGGAGAACAAAACTCTGGGGCCTAAACCCAGTCTGTTTGCAATTAATAAATGCTACAGCTCACCTGGGGCTCTGCTATGGAC

Tnf

NM_013693.1

TTCCTGAGTTCTGCAAAGGGAGAGTGGTCAGGTTGCCTCTGTCTCAGAATGAGGCTGGATAAGATCTCAGGCCTTCCTACCTTCAGACCTTTCCAGACTC

Tnfrsf1a

NM_011609.2

CTCCTTGCCAAGCTGACAAGGACACGGTGTGTGGCTGTAAGGAGAACCAGTTCCAACGCTACCTGAGTGAGACACACTTCCAGTGCGTGGACTGCAGCCC

Tnfrsf1b

NM_011610.3

GTGTGTGTCCATGTTTGCATGTATGTGTGTGCCAGTGTGTGGAGGCCAGAGGTTGGCTTTGGGTGTGTTTGATCACTCTCAGTTACTGAGGCAGGGCTCT

Trem2

NM_031254.2

GGGCGCCTACCCTAGTCCTGACTGTTGCTCAATCCAGGAGCACAGTTCCTGTGGGCTGAGCCTGACTGGCTTGGTCATCTCTTTTCTGCACTTCAAGGGA

Tspo

NM_009775.4

GACACTGGCTCCCATCTGGGGCACACTGTATTCAGCCATGGGGTATGGCTCCTACATAGTCTGGAAAGAGCTGGGAGGTTTCACAGAGGACGCTATGGTT