Fig. 4

Involvement of PAR2 in mediating MIA-induced joint inflammation. a The MIA-induced increase in knee joint diameter on day 1 post-injection was blocked by systemic treatment with GB83 (5 μg i.p., administered 10 min before and 120 and 240 min after MIA, on day 0 and once on day 1 (60 min before assessment) and absent in PAR2 knockout (PAR2^−/−) mice. b The increased number of rolling leukocytes were reduced in PAR2 knockout mice. b The increased number of adherent leukocytes on day 1 was reduced by treatment with GB83 (5 μg i.p., administered 10 min before and 120 and 240 min after MIA, on day 0 and once on day 1 (60 min before assessment)) and absent in PAR2 knockout mice. (n = 8–9 per group) *P < 0.05, **P < 0.01, ***P < 0.001, compared to the MIA control, one-way ANOVA. c Lower panel shows representative intravital micrographs in the different cohorts of mice; red arrows indicate stained leukocytes