Brain ureido degenerative protein modifications are associated with neuroinflammation and proteinopathy in Alzheimer’s disease with cerebrovascular disease

Table 1 List of proteins that contain exclusive HCit residues in the particulate brain proteome of AD + CVD

Gene	Protein	Peptide	Protein residue^b	Secondary Structure^c	Stoichiometry^d
SPTAN1	Spectrin alpha chain non-erythrocytic 1	K^aHQLLEADISAHEDR	1699	–	50
ATP1A3	Sodium/potassium-transporting ATPase subunit alpha-3	K^aADIGVAMGIAGSDVSK	717	–	16
ACTB	Actin cytoplasmic 1	NSIMK^aCDVDIR	284	–	67
ATP6V0A1	V-type proton ATPase 116 kDa subunit a	K^aANIPIMDTGENPEVPFPR	74	–	22
ATP2B4	Plasma membrane calcium-transporting ATPase 4	K^aADVGFAMGIAGTDVAK	795	–	41
BASP1	Brain acid soluble protein 1	K^aTEAPAAPAAQETK	150	–	50
GFAP	Glial fibrillary acidic protein	SK^aFADLTDAAAR	260	Coil 2B	55

^aThe homocitrulline residue in the identified peptide
^bThe residue number in the protein sequence
^cWhen available, the location of the HCit residue in the secondary structure of the protein
^dThe stoichiometric ratio (percentage of occurrence) of HCit from the total peptides identified that contain the Lys site in the protein (unmodified + modified)

ISSN: 1742-2094