Fig. 4
From: Primary cilia modulate TLR4-mediated inflammatory responses in hippocampal neurons
LPS treatment decreased primary ciliary length in cultured hippocampal neurons. After exposure to LPS (100 ng/ml) for 1, 6, and 12 h, HT22 hippocampal neurons were stained (a) for ARL13B (red) and DAPI (blue) and ciliary length was measured via confocal imaging (b). Over 100 cilia were measured for each group. Protein levels of ARL13B were quantified by Western blotting (c) under control conditions (cont) and after LPS exposure. The data are normalized to β-actin and quantified and expressed as optical densities and are presented as the mean ± SEM of three independent experiments. ***p < 0.001. Scale bar = 20 μm in a; 10 μm in the corresponding inset images