Fig. 2
Intrathecal injection of AM1241 (100pM) attenuated CCI-induced hyperalgesia and increased expression of P2Y12 and P2Y13R in the rat dorsal spinal cord. a RT-PCR quantitative analysis of the P2Y12R mRNA expression. *P < 0.05 means comparison with the sham group; + P < 0.05 means comparison with the vehicle-treated CCI group. n = 8 per group. b RT-PCR quantitative analysis of the P2Y13R mRNA expression. *P < 0.05 means comparison with the sham group; + P < 0.05 means comparison with the vehicle-treated CCI group. n = 8 per group. c Western blotting image of P2Y12R expression. The top panel was the target band, P2Y12R protein, and the bottom one was for the loading control beta-actin. d Western blotting image of P2Y13R expression. The top panel was the target band, P2Y13R protein, and the bottom one was for the loading control beta-actin. e Western blotting quantitative analysis of the P2Y12R expression. *P < 0.05 means comparison with the sham group; + P < 0.05 means comparison with the vehicle-treated CCI group. n = 8 per group. f Western blotting quantitative analysis of the P2Y13R expression. *P < 0.05 means comparison with the sham group; + P < 0.05 means comparison with the vehicle-treated CCI group. n = 8 per group