Fig. 4

CD16 expression level of ex vivo monocytes. PBMCs were stained with cell-type markers (CD3, CD19, CD14, CD16, CD56, CD66b) to analyze the monocyte subset population of each group. Results are shown in a as representative graphs and b as a large-scale analysis to compare three groups (n = 20). Confirmation of manual gating was performed using the SPADE 3.0 program. The bubble around the nodes represents a particular cell type that was selected according to expression of its lineage marker, as indicated by the color code (with expression decreasing from red to blue) (c). NMOSD shows particular increase in CD16 expression compared to MS and HC. Graphs were created using the Flowjo program, and bar graphs were created using Prism software. Assessment of statistical significance was performed by two-way ANOVA. Data are mean ± S.E.M of 20 subjects each. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001