Fig. 4

Hcy induced the secretion of proinflammatory cytokines TNF-α and IL-6 in ischemic brain injury. a, b Representative photomicrographs of the immunofluorescence staining of IL-6 (red) and TNF-α (green) in DG area of the hippocampus and the cerebral ischemic cortex. Nuclei are stained blue with DAPI. Each right-hand column depicts a magnified image of the rectangular region of the corresponding image in the left column. c-f Bar graphs show semi-quantification of fluorescence intensity for TNF-α and IL-6. Values are mean ± SD. Six rats in each group and 10 fields for each rat were examined. Bar = 50 μ m. g, h Western Blot analysis of TNF-α and IL-6. β-actin protein was used here as an internal control. The data are presented as the mean ± SD for five independent experiments. ^a p < 0.05 vs. the SHAM group, ^b p < 0.05 vs. the MCAO group