Fig. 4
Characterization of astrocytes derived from hSOD1G93A mice and from their non-transgenic littermates. a Representative immunofluorescence images showing FA (red) and stress fibers (green) in non-transgenic and hSOD1G93A-derived astrocytes in the presence or absence of Thy-1-Fc stimulation. Nuclei appear in red. Magnification bar = 50 μm. b Quantification of wound-healing assays on non-transgenic and hSOD1G93A-derived astrocytes treated with Trail-R2-Fc, Thy-1-Fc, Thy-1(RLE)-Fc, or serum (FBS). c Western blot of β3 Integrin, Syndecan-4, Connexin-43, Pannexin-1, P2X7R, and mutant hSOD1 protein levels in hSODG93A-derived astrocytes. Values shown are the means ± s.e.m. from three independent experiments. **p < 0.01; ***p < 0.001