Fig. 1
From: Inflammation-associated regulation of RGS in astrocytes and putative implication in neuropathic pain
Mechanical hypersensitivity measurement and spinal RGS expression after a SNI. Pain hypersensitivity was determined for the ipsilateral (a) and contralateral (b) hind paws at baseline (BL; before surgery) and during 3 weeks after sham surgery or SNI. PWT 50% after von Frey filament stimulation was used as a readout for allodynia and calculated as previously described [37]. Data are presented as mean ± SEM; n = 10 (0–14 days) or n = 5 (14–21 days); two-way ANOVA with Bonferroni’s post test; *p < 0.05, ***p < 0.001 (SNI vs sham). RGS mRNA expression, normalized to GAPDH expression, was then measured in the ipsilateral side of the lumbar spinal cord 3, 5, 7, 14, and 21 days after the SNI lesion and compared to sham-operated animals (c–f). Data are presented as mean ± SEM; n = 4; one-way ANOVA with Dunnett’s post test; *p < 0.05, **p < 0.01, ***p < 0.001 (vs sham). Data shown were obtained from independent experiments either for 3, 5, and 7 days or for 14 and 21 days. Sham, 3, 5, and 7 days: F 3,13 = 2393 (RGS2), 6178 (RGS3), 3434 (RGS4), and 7916 (RGS7). Sham, 14 and 21 days: F 2,15 = 16.83 (RGS2), 0.4426 (RGS3), 0.4370 (RGS4), 17.60 (RGS7)