Fig. 2

RGS expression and cell morphologies in astrocyte cultures exposed to different maturation conditions. After 17 days in 10% FBS-medium, astrocytes were exposed to FBS 3%-medium alone (a, b) or complemented either with the G5 supplement (c, d) or dBcAMP 150 μM (e, f). Immunostaining was performed using an antibody against GFAP coupled to Cy3 (red) and DAPI staining (blue) was achieved for the nuclei. As highlighted by the white shape, astrocytes adopted a typical protoplasmic (b) or stellate (d, f) morphology following the maturation condition. Bar, 200 μM. For every maturation condition, RGS mRNA expression was then measured by qPCR in independent experiments (g–j). Expression was normalized to GAPDH expression. Data are presented as mean ± SEM; n = 6; ANOVA one-way with Bonferroni’s post test; *p < 0.05, **p < 0.01, ***p < 0.001 (vs FBS 3%), ^### p < 0.001, ns indicates not significant. F _2,22 = 17.31 (RGS2), 37.70 (RGS3), 56.36 (RGS4), 14.41 (RGS7)