Fig. 2

MIA-induced an inflammatory milieu in prenatal/neonatal brain (a, b) and postnatal amygdala (c, d) in offspring. Expression of inflammatory markers and toll-like receptors (TLRs) in prenatal/neonatal brains and postnatal amygdala (a–d). TLR-4 and TLR-2 mRNA expression profiles were elevated in the developing brain and remained significantly higher up to 40 days post insult. Gene expression was normalized against β-actin via ∆Ct. Results are mean normalized to expression ± SEM (n = 4/treatment group/time point). Significant difference denoted as *p < 0.05, **p < 0.01, and ***p < 0.001 in post hoc multiple comparisons performed using Tukey’s test following one-way ANOVA