Fig. 4
From: DHCR24 exerts neuroprotection upon inflammation-induced neuronal death
APP, BACE1, and Aβ40 levels are not affected by DHCR24 overexpression or neuroinflammation in the neuron-BV2 cell co-cultures. a Western blotting was used to detect the levels of mature (APP695m), immature (APP695im), and total APP (APPtot, calculated as APP695m + APP695im). There were no significant differences in the levels of APPm, APPim, and APPtot. However, the ratio between mature and immature APP695 forms was significantly increased in the LPS and IFN-γ-treated control co-cultures. An APP fragment of approximately 60 kDa (APP*) was detected in the LPS and IFN-γ-treated samples, probably resulting from caspase-mediated cleavage of APP. DHCR24 overexpression did not affect the levels of this fragment. Molecular masses in kilodaltons are indicated on the left side of the blots. APP751 isoform derived from glial cells is indicated as with the asterisk (*) above the APP695m and APP695im bands. b The levels of BACE1 normalized to neuronal viability are increased upon LPS and IFN-γ treatment, but DHCR24 overexpression has no effect on BACE1 levels. c Aβ40 was detected from conditioned media using ELISA. Decreased levels of Aβ40 were detected in the LPS and IFN-γ-treated samples. This was possibly due to neuronal loss in these samples as normalization of Aβ40 levels to neuronal viability abolished the effect. Aβ40 levels were not significantly affected by DHCR24 overexpression. *p < 0.05, **p < 0.01, mean ± SEM, n = 6