Fig. 7

Minocycline treatment improves the proliferating, differentiation, maturating, and growth factor proteins following SD. a Identification of the DG region of the hippocampus. Representable image of b Ki-67. c BrdU cells in DG region of the hippocampus. Changes in the cell count of the d Ki-67. e BrdU cells in DG region of the hippocampus. Representable image showing f different stages of DCX positive cells in the DG region of hippocampus. g DCX expression among different groups in the DG region of the hippocampus. h Changes in the cell counts of DCX cells in the DG region of the hippocampus at proliferative, intermediate, and post-mitotic stage. i Representable image showing NeuN expression in the DG region of the hippocampus. j Changes in the relative mean pixel intensity of NeuN in the DG region of the hippocampus. k Representable image of BDNF expression in DG, CA1, and CA3 region of the hippocampus. l Changes in the relative mean pixel intensity of BDNF protein in the DG, CA1, CA3 region of the hippocampus. *p < 0.05 when compared to control treated with vehicle. ^ϕ p < 0.05 when compared to sleep deprived treated with vehicle. Kruskal-Wallis with Dunn’s post hoc test was applied for statistical analysis of BrdU, Ki-67, and DCX cell count. One-way ANOVA with Bonferroni post hoc test was used for statistical analysis of NeuN and BDNF protein