MicroRNA expression changes in association with changes in interleukin-1ß/interleukin10 ratios produced by monocytes in autism spectrum disorders: their association with neuropsychiatric symptoms and comorbid conditions (observational study)

Table 2 Differences of microRNA (miRNA) expression in ASD subjects with high, normal, and low IL-1ß/IL-10 ratio

	miRNA numbers	miRNA numbers
	Upregulated	Downregulated
Comparison between ASD samples
High ratio^a,b vs. low ratio	21	0
	> 4-fold higher 3^a
	> 3-fold higher 3
High ratio vs. normal ratio	19	0
	> 4-fold higher 4
	> 3-fold higher 2
Low ratio vs. normal ratio	0	0
Comparison with normal controls
High ratio vs. controls	7	0
	> 3-fold higher 2
Low ratios vs. controls	1	2
		> 3-fold lower 1
Normal ratios vs. controls	0	1
All ASD samples vs. controls	0	0

^aHigh, normal, and low IL-1ß/IL-10 ratio groups were defined in the 1st paragraph of the “Results” section
^bNumbers of ASD samples submitted for miRNA sequencing are the following: high ratio group (N = 43), low ratio group (N = 18), and normal ratio group (N = 47). Among non-ASD control cells (N = 27), 23 samples revealed normal IL-1ß/IL-10 ratios, 2 samples revealed high ratios with > + 1 SD in 2 culture conditions, and 2 samples had low ratios with < − 1 SD in 2 culture conditions. Thus, ASD samples examined in this study revealed much higher frequency of high/low IL-1ß/IL-10 ratios, as compared to non-ASD control cells (p < 0.0005 by Fisher’s exact test)

ISSN: 1742-2094