MicroRNA expression changes in association with changes in interleukin-1ß/interleukin10 ratios produced by monocytes in autism spectrum disorders: their association with neuropsychiatric symptoms and comorbid conditions (observational study)

Jyonouchi, Harumi; Geng, Lee; Streck, Deanna L.; Dermody, James J.; Toruner, Gokce A.

doi:10.1186/s12974-017-1003-6

Journal of Neuroinflammation

Table 4 Cytokine production by purified ASD PBMo with high, normal, or low IL-1ß/IL-10 ratios

From: MicroRNA expression changes in association with changes in interleukin-1ß/interleukin10 ratios produced by monocytes in autism spectrum disorders: their association with neuropsychiatric symptoms and comorbid conditions (observational study)

Cytokine measured	Stimulant	ASD—high (N = 43)	IL-1β/IL-10 ratio ASD—normal (N = 47)	ASD—low (N = 18)	Non-ASD cells (N = 27)
IL-1ß^a	Medium	628.7 ± 455.9	420.8 ± 210.2	206.0 ± 191.2	306.2 ± 199.3
	LPS	2518.0 ± 1012.0	1968.0 ± 460.2	1407.2 ± 586.7	1968.2 ± 460.2
	Zymosan	2640.2 ± 958.0	2205.6 ± 715.2	1682.6 ± 951.3	1872.0 ± 692.1
	CL097	3470.2 ± 917.0	3008.8 ± 848.0	2836.2 ± 1126.3	2257.1 ± 1040.7
	ß-lactam	2452.2 ± 1056.3	2108.3 ± 713.6	1725.3 ± 924.6	1726.2 ± 566.9
IL-6^b	Medium	3701.5 ± 1746.2	3784.4 ± 1137.1⁸	2829.1 ± 1197^j	2892.2 ± 1258.0
	LPS	23,478.1 ± 9649.6	22,071.3 ± 10,171.0	24,177.8 ± 10,285.6	18,459.5 ± 10,120.8
	Zymosan	4945.1 ± 1406.1	5064.0 ± 841.9	5147.9 ± 1705.8	5050.3 ± 1068.3
	CL097	4025.3 ± 1335.0	4382.5 ± 988.2	5180.6 ± 2170.4	4111.3 ± 1556.5
	ß-lactam	4842.3 ± 1238.1	4780.5 ± 1048.8	5130.9 ± 1935.2	4641.6 ± 1184.9
TNF-α^c	Medium	95.7 ± 100.2	101.3 ± 139.3	49.0 ± 70.0ⁱ	73.7 ± 114.3
	LPS	478.9 ± 373.9	509.5 ± 411.5	196.8 ± 194.9ⁱ	363.8 ± 399.7
	Zymosan	716.4 ± 463.4	731.0 ± 546.0	309.0 ± 241.8⁶	774.4 ± 587.8
	CL097	1148.1 ± 900.3	1304.7 ± 843.2	889.4 ± 534.9^e	859.6 ± 685.2
	ß-lactam	1200.5 ± 859.9	1136.5 ± 681.8	689.2 ± 440.3⁶	1061.2 ± 565.5
IL-10^a	Medium	678.5 ± 411.1	789.6 ± 319.9⁸	741.8 ± 447.7	533.4 ± 301.8
	LPS	1195.0 ± 338.7	1444.9 ± 325.2⁸	1556.6 ± 465.5⁸	1239.8 ± 358.0
	Zymosan	851.9 ± 388.4	1144.6 ± 339.1⁸	1166.0 ± 545.0⁸	874.5 ± 381.1
	CL097	235.3 ± 197.5	683.4 ± 408.5⁸	924.8 ± 549.0⁸	491.4 ± 294.7
	ß-lactam	674.1 ± 345.9	994.7 ± 355.5⁸	1126.5 ± 549.0⁸	740.7 ± 412.0
TGF-ß^d	Medium	300.8 ± 232.5	452.5 ± 341.8	418.2 ± 232.6	421.1 ± 263.1
	LPS	301.3 ± 232.3	424.0 ± 332.3	407.2 ± 253.9	432.4 ± 266.6
	Zymosan	257.8 ± 212.2	360.1 ± 302.0	366.1 ± 216.7	375.5 ± 225.5
	CL097	192.0 ± 127.7	363.3 ± 316.4	370.1 ± 300.1	397.5 ± 280.7
	ß-lactam	245.0 ± 199.6	314.3 ± 201.4	403.7 ± 308.6	331.6 ± 182.7

^aAs expected, IL-1ß and IL-10 levels differ among the ASD cell groups with high, normal, and low IL-1ß/IL-10 ratios and non-ASD control cells (p < 0.005 by one-way ANOVA with the use of log-transformed data)
^bIL-6 level production without stimuli differed among the groups tested, reflecting a higher IL-6 production by ASD cells with high and normal IL-1ß/IL-10 ratios (p < 0.05 by one-way ANOVA with the use of log-transformed data)
^cNF-α levels differed among groups under LPS- and CL097-stimulated cultures by one-way ANOVA with the use of log-transformed data (p < 0.01 for LPS and p < 0.05 for CL097)
^dTGF-ß levels differed among groups under cultures without stimuli (p < 0.05) and under CL097-stimulated cultures (p < 0.005) by one-way ANOVA with the use of log-transformed data

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ISSN: 1742-2094

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