Fig. 1

Temporal profiles and cellular distribution of sEH in WT mouse brains subjected to ICH. a Immunoblots of sEH protein in normal brains and the liver tissue. b Representative immunoblots and quantitative data of sEH protein levels in the hemorrhagic hemispheres from ICH or sham-operated mice. Values are mean ± S.E.M.; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001 vs. sham group (n = 5–6 mice/group, one-way ANOVA). c Double-labeling shows colocalization of sEH protein with microglia (CD11b), astrocytes (GFAP), neurons (NeuN), and endothelial cells (CD31) in the perihematomal area of both the contralateral and ipsilateral hemispheres at 1 day post-ICH. White arrowheads indicate colocalization. The inset images represent higher magnification of the colocalization regions in the corresponding images. Sections were stained with DAPI (blue) to show all nuclei. The scale bar is 100 μm