Fig. 4

Intracellular mAb47 co-localizes with α-syn oligomers in astrocytes. Co-cultures were exposed to Cy3-α-syn oligomers or to Cy3-oligomers pre-incubated with mAb47. a Immunofluorescence with secondary antibodies directed towards mAb47 displayed the antibody internalization (green). The Cy3-α-syn signal (red) was weak, indicating low oligomer accumulation. Enhancing the Cy3-α-syn signal revealed intracellular co-localization of oligomers and mAb47 (blue = DAPI). b As a control, cells were exposed to non-fluorescent α-syn oligomers that had been pre-incubated with mAb47. Co-staining with antibodies to GFAP (white), mAb47 (green), and oligomeric α-syn (detected by mAb38F, red) revealed co-localization of mAb47 and oligomeric α-syn inside astrocytes. Scale bars = 10 μm