Fig. 7

Lysosomal-endosomal inhibition does not alter the antibody-mediated effect on oligomer accumulation. a Co-cultures were exposed to either Cy3-α-syn oligomers or to mAb47-treated oligomers for 24 h, in combination with the lysosomal inhibitors bafilomycin (+Baf) or chloroquine (+Chq). The α-syn accumulation was unchanged, both in the absence and presence of mAb47. Hence, chemical inhibition of the lysosomal pathway did not alter the mAb47-mediated reduction of α-syn accumulation. b Fluorescence quantification of Cy3-α-syn area and (c) intensity confirmed that the inhibitors did not reduce the antibody-mediated effect on α-syn accumulation. Scale bars = 10 μm. Data are presented as mean ± SD from three independent experiments and levels of significance were set to *P < 0.05, **P < 0.01, and ***P < 0.001