Fig. 2

Differential modulation of chemoattractants and proinflammatory cytokines in astrocytes by bryostatin-1 and JQ1. Astrocytes were either left uninfected or infected with VSV-G-pseudotyped HIV-1 for 7 days and then treated for 24 h with the listed LRA. Cell-free supernatants were collected to measure a, b CCL2, c, d IL-6, e, f IL-8, and g, h GM-CSF production by commercial ELISA tests. Each value of uninfected (a, c, and e) and HIV-1-infected (b, d, and f) astrocytes either left untreated or treated with the listed LRA is presented in fold change over untreated cells except for the secretion of GM-CSF where results of g uninfected and h HIV-1-infected LRA-treated astrocytes are presented in raw data because values were under the detection threshold for untreated samples. Each colored dot represents a different donor, and the grand mean is shown for each experimental condition. Asterisks denote statistically significant data as defined by the Student t test (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001)