Fig. 4

Bryostatin-1 modulates C3 mRNA expression and glutamate uptake by astrocytes. a Astrocytes were treated with JQ1, bryostatin-1, or JQ1 and bryostatin-1 for 4, 8, or 24 h. Total RNA was extracted to measure mRNA expression of C3 by RT-qPCR. Results from four different donors are presented in fold change over the untreated condition. b, c Astrocytes were either left untreated or treated with JQ1, bryostatin-1, or JQ1 and bryostatin-1. Glutamate levels in the supernatant were measured at 2, 4, 8, and 24 h post-treatment using a commercial glutamate assay kit. Data from one representative donor for the four time points (b orange: untreated, pink: JQ1, blue: bryostatin-1, green: JQ1 and bryostatin-1) and five donors for the 24 h treatment (c) are presented. Asterisks denote statistically significant data as defined by the non-parametric Friedman test followed by Dunn’s multiple comparison test (*P < 0.05 and **P < 0.01)