Fig. 3

Levels of glutamate (a, b) and carbonyl content (c, d) in the CX and HC tissue from animals with PM after vitB6 supplementation. Glutamate level is significant increased during infection in both tissues and vitB6 has an effective influence up to 20 h p.i.. Reduction of carbonyl content were significant only in the CX/20 h in PM/pre sup vitB6. In contrast, in the HC, the vitB6 is associated to the highest level of carbonyls. Values were transformed to percentage using the control group at 20 h post infection as 100%. Each column data expresses the mean percentage (± SEM) relative to control 20 h from at least three or more animals. Comparison between columns which show p < 0.05 were significant in Dunn’s post test. Groups for the CX: uninfected control animals (CTRL) 20 h (n = 3), uninfected control animals (CTRL) 24 h (n = 5), infected animals (PM) without vitB6 treatment 20 h p.i. (n = 5), infected animals (PM) without vitB6 treatment 24 h p.i. (n = 5), infected animals plus vitB6 treatment (PM/pre sup vitB6) 20 h p.i. (n = 9), and infected animals plus vitB6 treatment (PM/pre sup vitB6) 24 h p.i. (n = 9). Groups for the HC: uninfected control animals (CTRL) 20 h (n = 3), uninfected control animals (CTRL) 24 h (n = 3), infected animals (PM) without vitB6 treatment 20 h p.i. (n = 3), infected animals (PM) without vitB6 treatment 24 h p.i. (n = 3), infected animals plus vitB6 treatment (PM/pre sup vitB6) 20 h p.i. (n = 9), and infected animals plus vitB6 treatment (PM/pre sup vitB6) 24 h p.i. (n = 4)