Fig. 1

Mechanical allodynia and Iba1 expression in the TG following IANX. a The timing of IANX or sham operation (upper) and the changes in MHWT of the ipsilateral whisker pad skin for 15 days following IANX or sham operation (lower). The MHWT was normalized to the pre-IANX MHWT (100%). Error bars indicate SEM. **p < 0.01 vs. sham (n = 6 in each; two-way ANOVA with repeated measures followed by Bonferroni’s multiple-comparison test). Iba1-IR cells in V1/V2 (b) and V3 (c) in the ipsilateral TG on day 3 following IANX or sham operation. Arrows indicate FG-labeled neurons. Arrowheads indicate Iba1-IR cells. d The relative area of Iba1-IR cells in V1/V2 and V3 in TG on day 3 following IANX and sham operation. Error bars indicate SEM. **p < 0.01 vs. sham. (n = 5 in each, Student’s t test). e The relative amount of Iba1 protein in TG on day 3 following IANX or sham operation. Error bars indicate SEM. *p < 0.05 vs. sham (n = 10 in each, Student’s t test)