Fig. 2

Effect of intra-TG LCCA administration on mechanical allodynia and Iba1 expression following IANX. a Photograph (upper) and photomicrograph (middle) of TG on day 3 following DiI administration into the TG through the guide cannula and photomicrograph (lower) of TG in naive rat. Arrows indicate DiI labeling. b The timing of the cannula implantation, LCCA, or Cont-LCCA administration into the TG, IANX, or sham operation (upper) and the changes in MHWT of the ipsilateral whisker pad skin following daily intra-TG LCCA or Cont-LCCA administration following IANX (lower). The MHWT was normalized to the pre-IANX MHWT (100%). Arrows indicate the timing of intra-TG LCCA or Cont-LCCA administration. Error bars indicate SEM. **p < 0.01 vs. sham (n = 6 in each; two-way ANOVA with repeated measures followed by Bonferroni’s multiple-comparison test). c Iba1-IR cells and FG-labeled TG neurons in V1/V2 on day 3 following intra-TG LCCA or Cont-LCCA administration in IANX rats. Arrows indicate FG-labeled neurons. Arrowheads indicate Iba1-IR cells. d The relative area of Iba1-IR cells in V1/V2 in TG on day 3 following intra-TG LCCA or Cont-LCCA administration in IANX rats. Error bars indicate SEM. **p < 0.01 vs. sham. (n = 5 in each, Student’s t test)