Fig. 5

The LPAR5/PKD axis controls the phosphorylation of pro-inflammatory transcription factors. a PMM were seeded on 12-well plates, serum-starved, and incubated with DMSO, DMSO plus LPA (1 μM), and LPA (1 μM) in the presence of TCLPA5 (5 μM) or CRT0066101 (1 μM) for the indicated time periods. The phosphorylation of p65-NF-κB, STAT1, STAT3, and c-Jun was detected by western blotting. One representative blot is shown (N = 3). b Densitometric analysis of western blots (N = 3). Results are presented as mean values + SEM (***p < 0.001, compared to DMSO-treated cells; ^## p < 0.01, ^### p < 0.001, LPA plus TCLPA5 or CRT versus DMSO + LPA; one-way ANOVA with the Bonferroni correction)