Fig. 5

Poldip2 mediates IκB upregulation in vivo and in vitro. a Baseline IκB mRNA was evaluated by quantitative RT-PCR in uninjured brains from Poldip2^+/+ and Poldip2^+/- mice. Bar graph represents means ± SEM from five mice per group normalized to GAPDH. **p < 0.01. b Astrocytes were transfected with siNegative (siNeg, non-silencing control) or siPoldip2 before exposure to TNF-α for 15 min. IκBα protein level was measured by western blotting and densitometry. Downregulation of Poldip2 after siRNA transfection was verified by western blotting. Bars represent means ± SEM of four to six independent experiments normalized to β-tubulin. Two-way ANOVA **p < 0.01 vs. siNeg no TNF-α