Fig. 3

Tenuigenin prevented dopaminergic neuron degeneration and NLRP3 inflammasome activation induced by MPTP in the SNc of mice. a Immunohistochemical staining of TH-positive neurons in the SNc of each group. b Stereological counts of TH-positive cells in the SNc of each group. c–g Immunoblot analysis of NLRP3, pro-IL-1β, pro-caspase-1, cleaved caspase-1 (p20), and IL-1β in the substantia nigra of each group. n = 4 for each group. Data are expressed as mean ± SEM, one-way ANOVA, *p < 0.05 and **p < 0.01 vs. saline group. ^# p < 0.05 and ^## p < 0.01 vs. MPTP group. Scale bar represents 200 μm (upper) or 50 μm (under)