Fig. 10

WWL70 treatment reduced PGE₂ synthesis in CCI mouse sciatic nerve. The fresh sciatic nerves from mice 7 days post-CCI surgery were subjected to qRT-PCR analysis. The enzymes that contribute to the PGE₂ synthesis COX-2 (a) and PGES2 (b) were greatly increased in CCI vehicle group and reduced by WWL70 (10 mg/kg). *p < 0.05. The increased expression of EP2 and EP4 receptor in the CCI vehicle mice was also attenuated by WWL70 treatment (c, d; *p < 0.05 and ***p < 0.001; mean ± S.E.M., n = 10). PGE₂ production was measured by EIA assay. Significantly increased PGE₂ in the vehicle group was remarkably blocked by WWL70 administration ((e), *p < 0.05 and **p < 0.01; mean ± S.E.M., n = 7)