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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface—the protective role for BDNF

Fig. 2

Evaluation of median eminence blood-brain barrier integrity. a Schematic representation of the protocol employed for evaluation of BBB integrity; in the enlarged scheme of the median eminence (ME) (right hand, bottom), FITC-dextran is shown as small green circles; in the case of barrier integrity, FITC-dextran is retained out of ME, whereas the loss of integrity allows transposition of FITC-dextran into the ME and neighboring areas. b Confocal microscopy analysis of FITC-dextran endogenous fluorescence in the region of the ME; in all acquisitions, the same settings of the microscope were employed (laser 488, wavelength = 405, %laser = 20%, gain = 1015, offset = − 0.3799). c Fluorescence in the ME was determined using an ImageJ software and presented as relative to control. N = 4; *p < 0.05 vs. control. 3v third ventricle, CTR control fed chow, HFD high-fat diet, ME median eminence, wk week

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Journal of Neuroinflammation

ISSN: 1742-2094