Transcriptional profile and Epstein-Barr virus infection status of laser-cut immune infiltrates from the brain of patients with progressive multiple sclerosis

Table 1 Summary of demographic and clinical data of MS cases, brain sample characteristics, and type and number of laser-cut immune infiltrates analyzed in this study

MS case/no. of brain tissue blocks analyzed	Sex/age at death	Disease duration (years)	Cause of death	Postmortem delay (hours)	RNA integrity number	Laser-cut samples used for gene expression analysis
MS case/no. of brain tissue blocks analyzed	Sex/age at death	Disease duration (years)	Cause of death	Postmortem delay (hours)	RNA integrity number	No. of meningeal infiltrates	No. of WM perivascular infiltrates	No. of total infiltrates
MS79/3	F/49	21	Bronchopneumonia, MS	7	6–7	3	3	6
MS92/3	F/37	17	MS	26	6.1–7.8	5	8	13
MS121/2	F/49	14	MS	24	6.8, 7.0	n.a.	7	7
MS154/2	F/34	11	Pneumonia	12	6.4, 8.3	2	3	5
MS160/2	F/44	15	Aspiration pneumonia, MS	18	6.0, 6.4	2	n.a.	2
MS176/1	M/37	27	Intestinal obstruction, MS	12	6.7	1	4	5
MS180/3	F/44	18	MS	9	6.4–6.7	7	9	16
MS234/3	F/39	15	Pulmonary embolism, pneumonia	15	7.4–7.9	6	n.a.	6
MS330/1	F/59	40	Pneumonia, MS	21	7.8	2	n.a.	2
MS402/1	M/46	20	Bronchopneumonia, MS	12	7.2	1	1	2
MS407/3	F/44	19	Septicaemia, pneumonia	22	6.2–6.7	7	4	11
No. of laser-cut samples analyzed						36	39	75

All brain tissues analyzed were from persons who died during the progressive phase of MS. The samples used for gene expression analysis include 36 samples from the meninges of 10 MS cases, of which 25 were B cell follicle-like structures and 11 were diffuse infiltrates; 39 samples from the white matter of 8 MS cases, of which 13 perivascular infiltrates were isolated from active lesions, 8 from chronic active lesions, 3 from areas of rarefied myelin with activated microglia, and 15 from non-demyelinated areas with activated microglia
n.a. not available

ISSN: 1742-2094