Fig. 3
Hypercapnia enhances activation of NLRP3 inflammasome in the hypoxic hippocampus (n = 4). a The immunoreactive bands of NLRP3 (118 kDa), pro-caspase-1 (40 kDa), caspase-1 (10 kDa), and GAPDH (36 kDa). c–e There are significant interaction effects between hypercapnia treatment and hypoxia treatment (NLRP3: P < 0.01; pro-caspase-1: P < 0.01; caspase-1: P < 0.05). b Simple effects analyses show that the protein expression levels in hypercapnia group has no significant difference compared with S group (ns P > 0.05). The protein expression levels in hypoxemia group are significantly increased compared with S group (**P < 0.01). HH group has the highest levels of the protein in comparison with hypercapnia group (**P < 0.01) and hypoxemia group (**P < 0.01). ns non-significant, S group sham-operated group, HH group hypercapnia + hypoxemia group. The concentrations of CO2 and O2 in the air are 0.03 and 21%, respectively