Fig. 7

Chronic minocycline treatment dampened microglial activation, NLRP3 inflammasome, and pro-inflammatory mediators in rats exposed to CMS. a Western blot analysis of Iba-1 (n = 4/group). b Western blot analysis of CD11b (n = 4/group). c–e PCR analysis of NLRP3, ASC, and caspase-1 (n = 4/group). f, g Western blot analysis of cleaved caspase-1 and cleaved IL-1β (n = 4/group). h–j PCR analysis of IL-1β, IL-18, and IL-6 (n = 4/group). All data are expressed as the mean ± SEM. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared to saline-treated rats. *p < 0.05, **p < 0.01, ***p < 0.001, compared to normal rats