Fig. 5

Genotype Affects Cellular Migration. One million macrophages suspended in serum-free media were placed in a 5-μm membrane separated from the chemoattractant, FBS, located in the bottom well. The cells that migrated across the membrane were lysed with a fluorescence lysis buffer. Fluorescence was quantified on a fluorescent plate reader at excitation/emission wavelengths of 490/520 nm. Data represent the mean ± SEM from three independent experiments, each performed in triplicate. Statistical significance was measured using an unpaired t-test with Welch’s correction. *p < 0.05