Fig. 4

Effects of IL-1RA and IL-1RA-PEP on transient MCAO-induced changes in tight junction protein expression and localization. a–e Protein expression levels of ZO-1, occludin, and claudin-5 in cerebral tissue of the control, vehicle, IL-1RA, and IL-1RA-PEP groups were evaluated. f–i Localization of ZO-1, occludin, and claudin-5 in the cerebral tissue of different groups was also analyzed. Scale bar = 50 μm. Data are shown as mean ± SEM; *P < 0.05, **P < 0.01 compared to the vehicle group, #P < 0.05 compared to the IL-1RA group, n = 4 per group, based on one-way ANOVA with Bonferroni correction. MCAO middle cerebral artery occlusion