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Table 1 List of primary antibodies

From: Evidence for the activation of pyroptotic and apoptotic pathways in RPE cells associated with NLRP3 inflammasome in the rodent eye

Antigen

Antibody

Dilution

Source

Applications

Amyloid-beta amino acid 1-16 (Aβ1–16)

Mouse monoclonal anti-Aβ1–16 (clone 6E10)

1:2000

1:500

BioLegend, Dedham, MA

Western blot

Immunohistochemistry

Caspase-1

Rabbit monoclonal

1:300

Abcam, Cambridge, UK

Immunohistochemistry

Phosphorylated NF-κB p65 (Ser 276)

Rabbit polyclonal

1:75

Santa Cruz Biotechnology, Dallas, TX

Immunohistochemistry

Interleukin-18 (IL-18)

Rabbit polyclonal

1:100

1:1000

Santa Cruz Biotechnology, Dallas, TX

Immunohistochemistry

Western blot

Active caspase-3 (aCasp-3)

Rabbit monoclonal

1:1000

Cell Signaling Technology, Beverly, MA

Immunohistochemistry

X-linked inhibitor of apoptosis (XIAP)

Mouse monoclonal

1:1000

BD Transduction Laboratories, San Jose, CA

Western blot

Gasdermin D (GSDMD)

Mouse monoclonal

1:100

Santa Cruz Biotechnology, Dallas, TX

Western blot

GAPDH

Mouse monoclonal

1:10,000

EMD Millipore, Billerica, MA

Western blot