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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The impact of metallothionein-II on microglial response to tumor necrosis factor-alpha (TNFα) and downstream effects on neuronal regeneration

Fig. 1

Fluorescently labelled Metallothionein I/II (MT488) is taken up by neurons, astrocytes and microglia after needlestick injury. Immunohistochemistry using markers to label the neurons (SMI312), astrocytes (GFAP) and microglia (Iba-1) on frozen cortical sections collected from adult rat 4 days after receiving needlestick injury with either the A488 or MT488. The MT488, but not A488, was visible in neurons (a); higher magnification images shown in b and c, similarly, the astrocytes also appeared to take up the MT488 but not A488 (d); higher magnification images shown in e and f. In contrast, both A488 and MT488 were taken up by microglia (g); higher magnification images shown in h and i. The microglia that taken up A488 appear in a more amoeboid-like morphology when compared to the microglia receiving the MT488, which had a more ramified phenotype. (Scale bar in a, d and g = 50 μm; b, c, e, g, h and i = 12 μm)

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