Fig. 2

Ppp3cb deficiency is associated with a decrease in GFAP-positive cells in the ARC, DMH, and VMH of HFD-fed mice. Immunohistochemical stainings for GFAP in the arcuate nucleus (ARC), dorsomedial hypothalamus (DMH), and ventromedial hypothalamus (VMH) of HFD-fed Ppp3cb WT and KO mice. Representative images of the mediobasal hypothalamus (a, b, upper pictures) and of astrocytes in the ARC, VMH, DMH (a, b, lower pictures) of HFD-fed Ppp3cb WT, and KO mice. Numbers of GFAP-positive cells in the ARC (c), VMH (d), and DMH (e). Length of primary projections in the ARC of Ppp3cb WT and KO mice (f). Numbers of primary projection per astrocyte in the ARC (g), VMH (h), and DMH (i). Means ± SEM; n = 6 biological replicates per genotype (four pictures per biological replicate were quantified) except for (d), where only five KO brains could be used and (f) where length could be measured only in four KO brains; unpaired two-tailed Student’s t test. *p < 0.05, **p < 0.01