Fig. 7

Enhanced activation of IL-1 receptor and Src leads to increased phosphorylation of GluN2B subunit at Ser1303 and reduced membrane-associated CaMKII in hyperammonemic rats. IL-1Ra or PP2 were added to hippocampal slices. CaMKII membrane association (a, b) and phosphorylation of GluN2B subunit at Ser1303 (c, d) were analyzed as described in the “Methods” section. Values are expressed as percentage of basal levels in control rats and are the mean ± SEM of 12, 15, 17, and 17 rats per group in a, b, c, and d respectively. Data were analyzed by two-way ANOVA. In a, F (1, 45) = 0.2872 for effect of HA, p = 0.5947; F (1, 45) = 1.742 for effect of IL-1Ra, p = 0.1936; and F (1, 45) = 7.909 for interaction, p = 0.0073. In b, F (1, 54) = 0.4278 for effect of HA, p = 0.5159; F (1, 54) = 18.16 for effect of PP2, p < 0.0001; and F (1, 54) = 8.633 for interaction, p = 0.0048. In c, F (1, 63) = 1.059 for effect of HA, p = 0.3073; F (1, 63) = 1.244 for effect of IL-1Ra, p = 0.2689; and F (1, 63) = 9.532 for interaction, p = 0.0030. In d, F (1, 63) = 1.701 for effect of HA, p = 0.1969; F (1, 63) = 2.920 for effect of PP2, p = 0.0924; and F (1, 63) = 7.765 for interaction, p = 0.0070. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. Bonferroni post-test: ^**p < 0.01, ^ap < 0.05, ^aap < 0.01, ^aaap < 0.001